Journal: Journal of Microscopy
Article Title: A method for the fast and photon‐efficient analysis of time‐domain fluorescence lifetime image data over large dynamic ranges
doi: 10.1111/jmi.13128
Figure Lengend Snippet: Comparison of F3‐CMM, LSM and conventional CMM on Rhodamine 6G data. (A) Diagram representing the set‐up of the capillary sample. Three rectangular capillaries filled with Rhodamine 6G and increasing concentrations of quencher KI were used. (B) Total intensity image obtained from the TCSPC data set. (C–E) Recovered lifetime images (left) and corresponding lifetime histograms (right). The histograms correspond to a strip of 67 pixels wide centred on each capillary. The lifetime scale is indicated as in (A). The background in the images represented an after‐pulsing in the range of 1–4%
Article Snippet: The dye and quencher mixture were freshly prepared and then used to fill up hollow rectangle capillaries (CM Scientific, ID 0.10 × 1.00 mm).
Techniques: Comparison, Stripping Membranes